Targeted DNA resequencing and gene expression analysis by high-throughput sequencing (constitutive and somatic mutation discovery)

The Genomics Core Facility is an IRCAN Facility dedicated to targeted DNA resequencing and gene expression analysis. It provides knowledge and access to cutting-edge, medium-to-high throughput tools and technologies, including high-throuput sequencing. It is open to all members of IRCAN, as well as to external academic or non-academic members of the scientific community. It is located at the 5th floor of the Pasteur tower.

identification of constitutive and somatic mutations by targeted resequencing (design of enrichment strategy, library preparation, high-throughput sequencing by semi-conductor technology, bioinformatic analysis)

Activity started mid-2012

Monot C, Kuciak M, Viollet S, Mir AA, Gabus C, Darlix JL, Cristofari G. The specificity and flexibility of l1 reverse transcription priming at imperfect T-tracts. PLoS Genet. 2013 May;9(5):e1003499 [IF 2014=7.5]

Richard-Fiardo P, Hervouet C, Marsault R, Franken PR, Cambien B, Guglielmi J, Warnez-Soulie J, Darcourt J, Pourcher T, Colombani T, Haudebourg T, Peuziat P, Pitard B, Vassaux G. Evaluation of tetrafunctional block copolymers as synthetic

vectors for lung gene transfer. Biomaterials. 2015 Mar;45:10-7 [IF 2014=8.6]

Albrengues J, Bourget I, Pons C, Butet V, Hofman P, Tartare-Deckert S, Feral CC, Meneguzzi G, Gaggioli C. LIF mediates proinvasive activation of stromal fibroblasts in cancer. Cell Rep. 2014 Jun 12;7(5):1664-78 [IF 2014=8.4]

Marsaud A, Dadone B, Ambrosetti D, Baudoin C, Chamorey E, Rouleau E, Lefol C, Roussel JF, Fabas T, Cristofari G, Carpentier X, Michiels JF, Amiel J, Pedeutour F. Dismantling papillary renal cell carcinoma classification: The heterogeneity of genetic profiles suggests several independent diseases. Genes Chromosomes Cancer. 2015 Jun;54(6):369-82 [IF 2014=4.0]